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To imitate hemorrhage toxicity in vitro , the model was constructed by applying 100 M hemin to Astrocytes ( Synthetic RNA Oligonucleotides and Transfection We purchased miRNA-146a-3p mimics, miRNA-146a-3p inhibitors, and a non-sense sequence for use as a miRNA negative control (NC) from Genepharma (Shanghai, China) (Supplementary Table 2)

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NNMT sits between NAD+ metabolism and one-carbon methylation metabolism, pathways that support redox reactions, DNA repair, and transcriptional regulation