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Supplies Needed Plan based on a 4-week protocol: Peptide Vials: 4 weeks 1 vial (70mg) Insulin Syringes (U-100): 28 syringes (1 per day) Bacteriostatic Water: 3 mL required 1 10 mL bottle Alcohol Swabs: 56 swabs recommend 1 100 pack Protocol Overview Goal: Investigate synergistic peptide activity in tissue repair, collagen signaling, and cellular regeneration pathways Schedule: Daily dosing for 4-16 weeks Follow with 24 week break Dose: 2.33 mg daily total blend Reconstitution: 3.0 mL per vial Storage: Lyophilized vials frozen

The PCR-amplified products were gel-purified, cloned into TOPO TA vector (Invitrogen TM ), and transformed into Escherichia coli PMC103 competent cells