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The column was eluted for 2 min from 0% B to 2% B (linear), from 3 to 6 min with a linear gradient from 2% solvent B to 3% solvent B, then isocratically from 3 to 18 min using 3% solvent B, 18 to 35 min with a linear gradient from 3% solvent B to 40% solvent B, 35 to 60 min using a linear gradient from 40 to 55% solvent B, then isocratically from 60 to 65 min at 55% solvent B then from 65 to 80 min from 55 to 0% B (linear) followed by equilibration from 80 to 90 min at 0% B

Vita-Complex Essential B-complex vitamins Designed to support the transformation of food into energy while also promoting a radiant and healthy complexion

Dec 2018;45(6):1173-1177

It is of interest to researches for its ability to improve bone density, reducing scaring, fight the effects of dementia, reduce seizure activity & improve renal function