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To corroborate the reproducibility of the LC-MS system, 10 L from extracted samples was pooled to produce quality control (QC) samples

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People with low B12 levels often report: Excessive daytime sleepiness Fatigue despite adequate sleep duration Various neurological symptoms that may indirectly affect sleep quality Correcting B12 deficiency typically improves these symptoms over several weeks to months, as the body's stores are replenished and metabolic function normalises