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The most immediate risk is dangerously low blood sugar, and strong IGF-1 signaling raises concerns about unregulated tissue growth

Generally speaking, women should aim for about 25 grams daily while men should aim for 30-35 grams daily, according to the Academy of Nutrition and Dietetics

cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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