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The number of years of life lost is calculated for each death which occurs between the ages of 35 and 74 years, and the number is multiplied by the smoking attributable fraction to give an estimate of the number of years of life lost attributable to smoking

The incubation system contained 1 mg BSA and 50 l of smoke solution obtained from cigarettes with or without charcoal filter in a final volume of 200 l of 50 mM potassium phosphate buffer, pH 7.4

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The most common pack size is 25 cigarettes, although there are still packs with 20 and even 30 cigarettes