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Nicotine was analyzed by GC/FID using an HP 5890 gas chromatograph with separation on an Agilent CP Wax 52 CB column (30 m 0.25 mm 0.25 m) using helium as the carrier gas with a flow rate of 1.2 mL/min

Abstract The retention of occupational and environmental particles in the lung is a primary determinant of biological effects