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The whole seedlings were homogenized at 24 h after the treatment, and the total RNA was extracted using the TRNzol formulation (TIANGEN, China)

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Three Signature Blend Recipes Evening Relaxation Blend 50% mullein leaf (smooth base) 25% damiana (gentle relaxation, mood support) 15% lavender buds (calming aromatic) 10% rose petals (smooth, sweet finish) Dream Work Blend 45% mullein leaf (neutral base) 25% mugwort (dream enhancement, visionary) 15% damiana (mood elevation) 10% lavender buds (calming accent) 5% marshmallow root (smoothness, binding) Refreshing Day Blend 60% mullein leaf (light base) 20% peppermint (cooling, energizing menthol) 15% damiana (gentle mood lift) 5% marshmallow root (smooth, extended burn) For more advanced blending techniques and additional recipe ideas, our dedicated herbal smoking blends recipe guide covers flavor pairing theory, seasonal variations, and ceremonial preparation methods in detail