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For the phosphorylated proteins, 1,000 l each of SuperSignal West Femto Luminol/Enhancer Solution (Thermo Scientific) and SuperSignal West Femto Stable Peroxide Buffer (Thermo Scientific) were mixed and pipetted onto the nitrocellulose membrane
In our study, the complication rate within one year after surgery was higher, 0.99 for continuing smokers and 0.67 for those who quit smoking due to the intervention
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