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Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps

Health Risks: Why the Comparison Matters Many people assume cigars are safer than cigarettes because theyre smoked less frequently or without inhaling

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Target Audience Target Audience Tobacco Manufacturers - Large and medium-sized companies producing cigarettes, cigars, heated tobacco products (HTPs) and smokeless tobacco