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Central Nervous System Effects Study Type: fMRI brain imaging study Key Finding: Cagrilintide activates amylin receptor-rich regions in the area postrema and nucleus tractus solitarius, distinct from GLP-1R activation patterns

Methods Construct cloning The full-length human ghrelin receptor (residues 1366) was sub-cloned into pFastBac1 vector with an N-terminal haemagglutinin signal peptide (HA) and His10 tag followed by BRIL epitope, as well as LgBiT at the C-terminus to facilitate the protein expression and stability

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