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Methods: Using a pharmacological approach, we modeled the behavioral and neurochemical effects of subchronic (4-day) nicotine (60g/kg, i.v.) or fluoxetine (1mg/kg, i.v.) exposure in adolescent and adult male rats
5 The measurement of cotinine--a primary metabolite of nicotine with a half-life of 16 to 18 hours that is detectable in urine, saliva, or serum--is a reliable method of determining use of or exposure to cigarettes or other tobacco products over a period of 2 to 3 days
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Studies have shown that interventions that combine medicine with behavioral counseling (at least 4 to 8 sessions) are more effective than brief advice or usual care
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