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HP is classified into acute, subacute, and chronic categories and Th1 polarization plays a significant role in developing acute/subacute HP and stimulation of reversible granuloma formation

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After deparaffinization, antigen retrieval and permeabilization, the samples were blocked and probed with the rabbit polyclonal antibodies against loricrin, involucrin (both Abcam, UK), filaggrin, KLK7 and TG1 (all three Invitrogen, USA) and appropriate anti-rabbit secondary antibody conjugated with Alexa Fluor 555 (Invitrogen, USA)