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Continued research is needed to elucidate the underlying pathophysiological mechanisms, the potential genetic influence, and the response to new therapeutic regimens, in order to optimize clinical management of this disease

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Importantly, control cells, including BEAS-2B and human lung organoids, were passaged in parallel under the same culture conditions and duration as the WCSE-exposed cells, which allowed us to compare adaptive changes arising specifically from chronic smoke exposure rather than from prolonged culture alone

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