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After 1060 min at 35 C for recovery, slices were transferred into oxygenated aCSF containing (in mM): 125 NaCl, 2.5 KCl, 1.25 NaH 2 PO 4 , 2 CaCl 2 , 1 MgCl 2 , 26 NaHCO 3 , 15 Sucrose, and 10 Glucose (pH 7.2, 325 mOsm) at room temperature for the rest of the day and individually transferred to a recording chamber continuously perfused at 2 mL/min with oxygenated aCSF

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Ironically, hes only a casual smoker (hes smoked Dunhill Reds ever since seeing The Beatles do the same in the Get Back documentary), and it takes him a solid month to get through a pack