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Materials and methods Substrate and inoculum All experimental samples received inoculation with a culture enriched in a lab-scale thermophilic (60 ) seed tank reactor (5 L), initially fed with untreated cigarettes at a concentration of 50 g/L volatile solids (VS)

doi: 10.1016/S1471-4892(01)00045-5 6 BalsRHiemstraPS