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The membrane was blocked with 5% skim milk for 1 h at room temperature and incubated with primary antibodies Bax, Bcl-2, Keap1, Nrf2, ZO-1, E-cadherin (1:1000 dilution) overnight at 4 , followed by HRP-conjugated secondary antibodies for additional 1 h at 37

doi: 10.3389/fpubh.2025.1574623 Received 12 February 2025 Accepted 25 July 2025 Published 14 August 2025 Volume 13 - 2025 Edited by Rishabh Kumar Kumar Rana, Shaheed Nirmal Mahto Medical College and Hospital, Dhanbad, India Reviewed by Hajed Al-Otaibi, King Abdulaziz University, Saudi Arabia Hyunchan Lee, National Eye Institute (NIH), United States Mark A

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Research shows that 9.6 million adult smokers in the UK live with a mental health condition, and two in every 5 cigarettes in England are smoked by individuals with a mental health condition