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Cell types, including human fibroblasts, neutrophils, airway epithelial cells, human embryonic stem cells, and mouse neural stem cells, demonstrate morphological changes, even displaying cytotoxicity in stem cells and fibroblasts when exposed to undiluted e-liquid and flavor aldehydes, respectively (Sisson, 2007)

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A total of 193 were excluded as they had recently used another tobacco or smoking cessation product and 57 had incomplete data on their prior cigarette use, leaving a final sample of 3,609 who were exclusive current e-cig users and ex-cigarette smokers

For all experiments, a delay was allowed between filling the atomizer and vaporization to completely soak the mesh and thus avoid dry burn (and thus false results)