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A threshold cycle number (CT) for each sample was obtained from a Prime Pro 48 Real-Time PCR System (Techne, United Kingdom) and used to evaluate the relative amount of target mRNA in experimental groups with those of control subjects using the 2 CT method (Livak and Schmittgen, 2001

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The knowledge and perception statements were measured on a Likert scale, including strongly agree, agree, neutral, disagree, and strongly disagree

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