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Immunofluorescent staining was performed with a Mouse Monoclonal anti-Vimentin Ab (Clone Vim 3B4, Dakocytomation) 1:100 1 h RT followed by a secondary anti Mouse IgG (whole Molecule) Ab R-Phycoeritrin conjugate (P9287 Sigma) in the dark for 1 hour at RT

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XZ-confocal microscopy of airway surface liquid height To measure the height of the ASL, PBS (20 l) containing 2 mg/ml rhodamine-dextran (10 kDa

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