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Pyocyanin binding to eDNA Previous studies have demonstrated that eDNA is a key constituent in the construction and structural integrity of the biofilm matrix in many bacterial species, and the cleaving of eDNA by nuclease enzymes such as DNase I disintegrates the biofilm matrix, thereby increasing the susceptibility of bacterial cells within the biofilm matrix to antimicrobial agents such as detergents and antibiotics [33, 34]

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For instance, a protein may exhibit a single band in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) but present three bands in isoelectric focusing

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