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The threshold intensity was manually set and kept constant, and the number of pixels was determined for 4G8-, ThS-, Iba1- and GFAP stained sections to quantify amyloid load, fibrillar burden, microglial activation and reactive astrogliosis in hippocampal (CA1, CA2, CA3 and dentate gyrus), subiculum and cortical areas (including motor, somatosensory, visual, frontal, parietal and retrosplenial cortex)