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doi:10.1016/S0021-9258(19)40041-0 105

Protein was eluted with buffer A, containing 300 mM imidazole, then concentrated and digested overnight at 4 C with thrombin (Biopharma, 10 unit/mg protein)

However, surveys also show that a majority of employers overall still do not cover these medications for weight loss, often citing cost concerns

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