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Metaphases analysis To assess single-chromosome damage, HCT116 Wild type (WT) and ADH5 cells were plated in P60 dishes, allowed to adhere and then treated with mitomycin C (MMC, Santa Cruz, #sc-3514) 0.5 g mL 1 during 24 h or with FA 150 mol L 1 during 48 h

The exact amount depends on the medical issue being treated

Nano-carriers whose surfaces are designed to contain active ligands (cancer cell markers or antibodies) - binding structures, based on metals (such as systems carrying transferrin drugs), polymers (nanocapsules and nanospheres), or lipids (such as sulfur-containing glycoside nanoliposomes) carries ferroptosis inducers, which can be modified to target specific cancer cells and achieve anti-tumor effects (Neganova et al., 2022)