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PLoS One 15, e0225392e0225321
Research protocols employ diverse approaches including isolated mitochondria preparations, permeabilized muscle fiber respirometry, stable isotope tracers for metabolic flux analysis, cellular bioenergetics assays (Seahorse), and in vivo metabolic phenotyping in rodent models
The key driver of results is sustained supplementation over weeks rather than a single well-timed dose
10-13 Although pacing is a beneficial initial management strategy, pharmacologic options are often needed for effective symptom reduction
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