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pWZ111 contains the homology arms used for insertion of transgene at LGI site ttTi4348 , and a Self-Excising Cassette containing sqt-1p::SQT-1 and rps-0p::hygG flanked by loxN sites, and a hsp::Cre

Increased A and neurofibrillary tangle deposition in the brain subsequently promote further cerebrovascular dysfunction via increased ROS production and inflammation ( Systemic vascular structure and function Though it remains unclear whether reductions in CBF or cerebral metabolism occurs first in the ADRD cascade, mechanical factors such as systemic and cerebral arterial stiffening are additional early stage vascular markers implicated in cerebral hypoperfusion in older adulthood beyond the typical effects of aging (Mitchell, 2011

made in-house) were incubated overnight at 4C, followed by staining with donkey anti-rabbit Alexa Fluor 488 Plus antibody (1:1,000

Nonaka, N