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Human islets were transduced with 100,000 physical particles/IEQ in complete CMRL media as described before [19], and PBMCs were transfected with pX601_saCas9-mCherry_gRNA- CNR1 using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturers instructions
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This limited prophylactic activity has become widely accepted as the essential characteristic of standard cytoprotective agents
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