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The reactions of fatty acid biosynthesis and steroid biosynthesis utilize large amounts of NADPH

This therapeutic approach may enhance cognitive benefits and neuroprotective effects by improving brain insulin signaling and glucose transport

Refrigeration at 2-8C and use within 28 days is the documented storage protocol

Extended reaction time Reagent Preparation Prepare 4 N NaOH Add 15 mL water to flask Add 5.2 mL 50% NaOH (19.4 N) to 25 ml volumetric flask Mix Cool to room temperature Bring volume to 25 mL with water Store in a plastic bottle at room temperature Add 4 g NaOH pellets to 25 ml volumetric flask Add 20 mL water volumetric flask Mix until dissolved Cool to room temperature Bring volume to 25 mL with water Store in a plastic bottle at room temperature Prepare calibrators in a 2 to 1 MPA:Assay Buffer Solution 0 uM GSSG - MPA:Assay Buffer 5 uM GSSG - 25 uL calibrator concentrate in 975 uL MPA:Assay Buffer Solution 10 uM GSSG - 50 uL calibrator concentrate in 950 uL MPA:Assay Buffer Solution Note: Calibrators will be treated and assayed the same as the samples except they don't need to be frozen