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Cell Viability Assay HepG2 cells were plated on collagen-coated 96-well plates at the density of 1 10 4 per well in 0.1 mL DMEM

The problem of excess B12 started in the last decade with the availability of highly efficient oral and injectable forms of B12

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Treatment of the proteins with DTT prior to application to the array allowed us to distinguish between those proteins where BioGEE had been incorporated into the protein via a non-disulphide mechanism (possibly due to interaction with biotin) from those that formed disulphide bonds with the GSH in BioGEE and hence were more likely to represent potential targets of glutathionylation