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Consistent with its enzymatic role, FADS knockdown resulted in increased VB2 levels, decreased FAD levels, and no significant change in FMN levels (Fig

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Mouse embryonic fibroblast cell line NIH/3T3 (#CRL-1658, ATCC), 293 T cells (#CRL-3216, ATCC) and normal liver cell NCTC1469 (#CCL-9.1, ATCC) were cultured in DMEM medium (#6123047, Gibco) with 10% FBS