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The peptide itself is a modified alpha-melanocyte-stimulating hormone analog (alpha-MSH) composed of a precise amino acid sequence that is irreversibly damaged by mechanical agitation, temperature excursions above 8C after reconstitution, or bacterial contamination introduced during mixing

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Samples were then centrifuged at 800G for 5 min, and 200 L of the supernatant was retrieved and transferred into a 96-well black plate

The reactive arm identifies proteins that escape productive folding or become damaged over time and routes them into clearance or repair pathways, primarily the ubiquitinproteasome system and lysosomal autophagy (Fig