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DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water

The SpendTrend26 report by Discovery Bank and Visa already documents changes in eating habits in South Africa, and the Consumer Goods Forum identifies a reshaping of food decisions in Europe

Human G1 and G2 were inserted into a pFastDual plasmid and were prepared by insertion of His 6 -tagged human G 1 fragment (BamHI/HindIII site) under the polh promoter and insertion of G 2 fragment (XhoI/SphI site) under the p10 promoter, respectively (Genewiz)

Ethics declarations Competing interests Angela Mastaloudis was employed by Brassica Protection Products, LLC., Jed W