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The column was washed with 15 column volumes of PPS buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4) at a flow rate of 5 ml min 1
Grade II (moderate / partial tear) Goals Protect the injured ligament while beginning controlled movement Restore ROM Strengthen surrounding muscles Rebuild stability through proprioception Safely progress back to daily/sport use Typical plan Protection / acute phase (first 12 weeks) Use of a hinged knee brace (locked or limited motion) to protect the knee during early healing Crutches if needed (offload to reduce pain) Ice, compression, elevation Pain-free ROM exercises (within brace limits) Gentle muscle activation (quads, hamstrings) Early rehab (weeks 24) Gradually unlock the brace to allow controlled ROM Continue strengthening: seated knee extensions, hamstring curls, calf raises Closed-chain work (mini squats, partial lunges) Proprioceptive drills (balance, single leg stance, wobble pad) Manual therapy / soft tissue techniques on tight muscles Intermediate / loading phase (weeks 46 or more) Increase resistance of strength exercises Dynamic stability (lateral steps, single leg squats) Light jogging when pain and stability allow Sport- or activity-specific drills (cutting, pivoting) Return to sport / full activity (weeks 68+) Strength and hop testing (90 % of uninjured side) Agility, plyometrics, side steps Monitor for symptoms of instability Gradual full return Typically, a Grade II case can take 48 weeks to recover, though more demanding sports may require more time
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