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Although the concentrations of sugars, aldehydes, and oxidants in the experiments exceeded physiological levels, they were instrumental in simulating physiological processes occurring over weeks or even months in the human body within a relatively condensed timeframe

For instance, CR combined with radiotherapy, has been shown to modulate the TME in a triple-negative breast cancer model by decreasing the number of intratumoral Tregs, increasing the CD8 + cell: Treg ratio, and upregulating PD-1 expression on CD8 + T cells

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