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Transient transfection of the PX459-sgRNA into the target cancer cell lines was performed using the Lipofectamine 3000 Transfection Reagent (Invitrogen) in 6-well plates (Corning Falcon) followed by puromycin selection (Gibco, 15 g/ml) for 3 days

Annual peer-reviewed publications in journals such as BMJ, NEJM, Lancet Infectious Disease and more

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Bottom line: If your supplement isn't bioavailable, timing won't matter much