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Single cells were sorted into 96-well plates in complete RPMI 1640 medium and allowed to recover for 72 h before the addition of fresh media

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Peptide Blend Vials (12 mg each): 8 weeks (~11.2 mL total): 4 vials 12 weeks (~16.8 mL total): 6 vials 16 weeks (~22.4 mL total): 8 vials Insulin Syringes (U100): Per week: 7 syringes (1/day) 8 weeks: 56 syringes 12 weeks: 84 syringes 16 weeks: 112 syringes Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution