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Total weight loss from baseline to week 52 was 6.7% in the placebo group, 10.9% with exercise alone, 13.4% with liraglutide alone, and 15.7% with combination therapy

The following day medium was removed and replaced with 20 l of loading medium consisting of 1:1 (v/v) Opti-MEM: Hanks balanced salt solution (HBSS), 2.5% (v/v) FBS, 20 mM HEPES, pH 7.4, 2.5 mM probenicid and fluorescent indicator 2 M Fluo-4 AM (Invitrogen)

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