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Briefly, adult zebrafish were anesthetized in 0.02% tricaine solution until gill movements slowed, decapitated, and eyes enucleated and placed vitreal side down on filter paper (0.45 m pore size

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f , Fold change (log 2 ) in indicated whole cell or ER metabolites from ER-tag HEK293T cells treated with thapsigargin compared to untreated

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