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Notably, exposure to nicotine-free e-vapor induces significant metabolic alterations and liver injury in dams and progeny, whereas nicotine-containing e-vapor promotes hepatic steatosis in adult offspring [135]

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The membrane pellet was homogenized and solubilized by gentle nutation for 2 hours at 4 C in buffer consisting of 20 mM Tris-Cl (pH 8.0), 150 mM NaCl, 1% Dodecyl--D-Maltosid (w/v, DDM), and 20 M DNQX, followed by centrifugation at 200,000 g for 30 min to remove insoluble material