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Discontinuing allows pigmentation to gradually return
A simple rapid method to precisely determine 13 C/ 12 C ratios of plant methoxyl groups
After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)
Rotating between thigh, abdomen, and upper arm helps reduce skin irritation and the small bumps (lipohypertrophy) that can develop from injecting the same spot repeatedly
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