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Nearly 30% of patients taking Ozempic and insulin developed low blood sugar

HEK293 platA cells were transfected with pMXs-CIP4-mEos4b-IRES-HygR or pMXs-CIP4-mCherry-IRES-HygR using 293-fectin (Invitrogen) for 2 days to produce retrovirus, and the supernatant containing the retrovirus was filtered and infected with CIP4 KO PANC-1 cells in the presence of polybrene (Sigma, TR-1003) at 32 g/ml

To elucidate the in vitro release mechanism of ORI@Lipo and ORI@CPD-Lipo, their release profiles were fitted using zero-order, one-order, Higuchi, Ritger-Peppas, and Weibull models