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Cholesterol possesses a strong membrane fusion capacity, facilitating the internalization of NADs and their entry into the cytoplasm

Inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor- (TNF-), along with hypoxic stress, oxidative damage, and uremic toxins, have been shown to suppress the expression and activity of CYP27B1 through multiple signaling pathways, resulting in reduced synthesis of 1,25(OH)D ( In addition to hydroxylase dysregulation, another critical barrier to effective vitamin D signaling in CKD lies in the impaired stability of the VDR

For the blank group, PBS is used instead of the ABTS working solution

Ozturk A, Olson MC, Samir AE, Venkatesh SK