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Slides were mounted with Fluoromount-G (Southern Biotech, USA) and examined using a Leica TCS SP5 AOBS confocal laser scanning microscope (Leica Microsystems, Germany) equipped with HCX PL APO 63 oil (NA = 1.4) objectives

Introduction Cysteine, a sulfhydryl-containing proteinogenic amino acid essential for the human body, is employed in a variety of metabolic pathways, such as the regulation of reduced glutathione (GSH), a major endogenous antioxidant molecule and the generation of sulfur-containing biomolecules such as hydrogen sulfide (H 2 S), taurine, coenzyme A and biotin ( Figure 1 ) ( Figure 1 Cysteine metabolism in cancer and tumor microenvironment It is increasingly noted that altered levels of amino acids including cysteine in the tumor microenvironment, by uptake or processing of both intracellular and extracellular regions, have emerged as potential markers of tumor progression

Park SJ, Yim GW, Paik H, et al

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